The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, College of Missouri). The PCR products and solutions ended up digested and cloned right into a pET21a+ vector working with NdeI and XhoI restriction enzymes. A degree mutation was introduced https://leonardou009grd2.idblogmaker.com/profile